Efficient reamplification of differential display products by transient ligation and thermal asymmetric PCR
نویسندگان
چکیده
منابع مشابه
Efficient reamplification of differential display products by transient ligation and thermal asymmetric PCR.
A new method for specific reamplification of DDRT-PCR products is presented. After transient ligation of the primary DDRT-PCR fragments into a T-vector, the cDNAs of interest were reamplified by hemi-nested PCR and thermally asymmetric cycles. In contrast to the originally described protocol, this method of reamplification is specific, sensitive, reproducibly gives a high yield of DNA and allow...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1998
ISSN: 1362-4962
DOI: 10.1093/nar/26.4.1130